Equipment and Capabilities

Library Preparation Workflows

  • Standardized amplicon workflows (e.g., 16S approaches)
  • Validated primer/adaptor resources
  • Pre-plated primer systems
  • Infrastructure suitable for low-input and environmental samples

All laboratory work is performed by trained users.


Quantification

  • Qubit 4 Fluorometer
  • BioTek Synergy Plate Reader
  • Quant-iT assay kits available via recharge

Quality Control

  • Agarose gel electrophoresis
  • UVP Chemidoc imaging
  • Agilent Bioanalyzer (users provide kits)

PCR & qPCR

  • Thermocyclers (including gradient capability)
  • ABI StepOne Real-Time PCR
  • Bio-Rad CFX96 Real-Time PCR

Sample Preparation

  • TissueLyser II
  • Chemical fume hood for DNA/RNA extraction
  • UV crosslinker

*GTAC provides infrastructure and training to support users in generating sequencing-ready libraries but does not perform sequencing.